On the week of 10/6 we planned out the rest of our experiment that way we were prepared for the process. We figured out the salt concentration and decided on a serial dilution starting with 1M, to 0.1M, 0.01M, 0.001M concentrations. These concentrations are how we will be able to determine the effects of biofilm. The serial dilution started with an eppendorf tube filled with 1000 micro liters of stock salt. The picture below explains the concentrations in each.
9/14 and 9/15 labs
On 9/14 we did a gram stain on D. Rad. Unfortunately there were not enough cells and we did not heat fix long enough to see any result under the microscope. The cells began to wash away during the process. On 9/15 we decided to reattempt gram staining on P. fluorescens. This time we heat fixed even more so the cells did not wash away. The sample was a bit older coming from August, therefore the cells appeared as debris rather than clumps of cells.
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