On the week of 10/6 we planned out the rest of our experiment that way we were prepared for the process. We figured out the salt concentration and decided on a serial dilution starting with 1M, to 0.1M, 0.01M, 0.001M concentrations. These concentrations are how we will be able to determine the effects of biofilm. The serial dilution started with an eppendorf tube filled with 1000 micro liters of stock salt. The picture below explains the concentrations in each.
The week of 10/11 and 10/12, Becca and I filled our 96 well plates with 10 micro liters of salt and 90 micro liters of our TGY with Gobi. After this was done, we had to let the bio film grow for 4-5 days. After the growth the next steps were doing a water wash, the CV, and the acetic acid. The process for the water wash and Crystal Violet is to sling, wash with water, pat dry, and repeat 3 times.
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