After about 3-4 weeks, we have ran 3 trials for each salt concentration. We have one set of data points so far and by Tuesday we will have all 3 trials figured out. The first round of data points all turned out good with no contamination. The process has been good overall and we have not ran into any major issues. In the beginning we had a few hiccups with contamination on our TGY plates but nothing since. We are excited to get the rest of our data points and run the statistics to put a poster together.
9/14 and 9/15 labs
On 9/14 we did a gram stain on D. Rad. Unfortunately there were not enough cells and we did not heat fix long enough to see any result under the microscope. The cells began to wash away during the process. On 9/15 we decided to reattempt gram staining on P. fluorescens. This time we heat fixed even more so the cells did not wash away. The sample was a bit older coming from August, therefore the cells appeared as debris rather than clumps of cells.
That's awesome that you guys are making really good progress! It is also good that you guys will have data to create statistics and create a poster from your project.
ReplyDelete