The week of 10/11 and 10/12, Becca and I filled our 96 well plates with 10 micro liters of salt and 90 micro liters of our TGY with Gobi. After this was done, we had to let the bio film grow for 4-5 days. After the growth the next steps were doing a water wash, the CV, and the acetic acid. The process for the water wash and Crystal Violet is to sling, wash with water, pat dry, and repeat 3 times.
On 9/28 we took 3 mL of TGY broth and placed it in a 15 mL flacon tube to prepare for inoculation on Saturday. Chad did a 4 way streak to begin growing the Gobi and allow to inoculate over night. On 9/29 we heated up the TGY broth that has agar to begin plating. The plates had some growth visible but no individual colonies. We did a gram stain on the culture that grow to look for grape like clusters of cells. Rebecca took a cluster of cells straight from the plates into a slide that has 5 micro-liters of TGY broth. The gram stain showed gobi was present but the heat fix caused the cells to turn black instead of purple.
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