On 9/1 in lab we went over the gram staining process as well as viewed the results under a microscope. There were no complications other than some imperfections on the microscope slide. A large pink oval appeared when viewing. We also observed Chad running his protein gel. These gels are ran vertically rather than the gels we have ran in the past that were done vertically. It took 30 minutes to let the gel bands separate before cracking off the plastic and letting it sit on the rocker over the weekend. The most important tip Chad gave us was to make sure the gel did not fold on itself.
The week of 10/11 and 10/12, Becca and I filled our 96 well plates with 10 micro liters of salt and 90 micro liters of our TGY with Gobi. After this was done, we had to let the bio film grow for 4-5 days. After the growth the next steps were doing a water wash, the CV, and the acetic acid. The process for the water wash and Crystal Violet is to sling, wash with water, pat dry, and repeat 3 times.
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