On the week of 10/6 we planned out the rest of our experiment that way we were prepared for the process. We figured out the salt concentration and decided on a serial dilution starting with 1M, to 0.1M, 0.01M, 0.001M concentrations. These concentrations are how we will be able to determine the effects of biofilm. The serial dilution started with an eppendorf tube filled with 1000 micro liters of stock salt. The picture below explains the concentrations in each.


Comments

Post a Comment

Popular posts from this blog

Image
After about 3-4 weeks, we have ran 3 trials for each salt concentration. We have one set of data points so far and by Tuesday we will have all 3 trials figured out. The first round of data points all turned out good with no contamination. The process has been good overall and we have not ran into any major issues. In the beginning we had a few hiccups with contamination on our TGY plates but nothing since. We are excited to get the rest of our data points and run the statistics to put a poster together.
Read more
Image
The week of 10/11 and 10/12, Becca and I filled our 96 well plates with 10 micro liters of salt and 90 micro liters of our TGY with Gobi. After this was done, we had to let the bio film grow for 4-5 days. After the growth the next steps were doing a water wash, the CV, and the acetic acid. The process for the water wash and Crystal Violet is to sling, wash with water, pat dry, and repeat 3 times.
Read more

Photo-bacterium Agar Broth; Making Media

Image
3/10/18 For this week in lab, the initial process we learned was making a broth that would eventually have the bacterium added to it. We gathered all the materials and prepped 2 sizes of broth to create. The steps were simple as we weighed out each component carefully. The mixture was created then stirred on a hot plate with a stirring rod inside. This was then stored after proper labeling for later use. On the same day we performed a gram stain this process to me seemed to be one we will use often in lab. We followed the proper steps using heat fix then the 4 steps to staining. The results were the best part in which we saw results in the microscope. This was found to be gram positive.
Read more