On the week of 10/6 we planned out the rest of our experiment that way we were prepared for the process. We figured out the salt concentration and decided on a serial dilution starting with 1M, to 0.1M, 0.01M, 0.001M concentrations. These concentrations are how we will be able to determine the effects of biofilm. The serial dilution started with an eppendorf tube filled with 1000 micro liters of stock salt. The picture below explains the concentrations in each.
Photo-bacterium Agar Broth; Making Media
3/10/18 For this week in lab, the initial process we learned was making a broth that would eventually have the bacterium added to it. We gathered all the materials and prepped 2 sizes of broth to create. The steps were simple as we weighed out each component carefully. The mixture was created then stirred on a hot plate with a stirring rod inside. This was then stored after proper labeling for later use. On the same day we performed a gram stain this process to me seemed to be one we will use often in lab. We followed the proper steps using heat fix then the 4 steps to staining. The results were the best part in which we saw results in the microscope. This was found to be gram positive.
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