After about 3-4 weeks, we have ran 3 trials for each salt concentration. We have one set of data points so far and by Tuesday we will have all 3 trials figured out. The first round of data points all turned out good with no contamination. The process has been good overall and we have not ran into any major issues. In the beginning we had a few hiccups with contamination on our TGY plates but nothing since. We are excited to get the rest of our data points and run the statistics to put a poster together.
Photo-bacterium Agar Broth; Making Media
3/10/18 For this week in lab, the initial process we learned was making a broth that would eventually have the bacterium added to it. We gathered all the materials and prepped 2 sizes of broth to create. The steps were simple as we weighed out each component carefully. The mixture was created then stirred on a hot plate with a stirring rod inside. This was then stored after proper labeling for later use. On the same day we performed a gram stain this process to me seemed to be one we will use often in lab. We followed the proper steps using heat fix then the 4 steps to staining. The results were the best part in which we saw results in the microscope. This was found to be gram positive.
That's awesome that you guys are making really good progress! It is also good that you guys will have data to create statistics and create a poster from your project.
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