On 9/21 we researched background on biofilm to make sure this is what we wanted to work on. On 9/22 Rebecca and I formed a plan for our research project. We came up with the idea to use different concentrations of several metal ions to test the relation to growth of biofilm. We made a flow chart to map out the process and find an overview of our procedures. So far we have run into the problem of the days not matching with the lab schedules. Although we are off to a slow start, I feel like next week we will really be able to begin our project.
Photo-bacterium Agar Broth; Making Media
3/10/18 For this week in lab, the initial process we learned was making a broth that would eventually have the bacterium added to it. We gathered all the materials and prepped 2 sizes of broth to create. The steps were simple as we weighed out each component carefully. The mixture was created then stirred on a hot plate with a stirring rod inside. This was then stored after proper labeling for later use. On the same day we performed a gram stain this process to me seemed to be one we will use often in lab. We followed the proper steps using heat fix then the 4 steps to staining. The results were the best part in which we saw results in the microscope. This was found to be gram positive.
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