On 9/1 in lab we went over the gram staining process as well as viewed the results under a microscope. There were no complications other than some imperfections on the microscope slide. A large pink oval appeared when viewing. We also observed Chad running his protein gel. These gels are ran vertically rather than the gels we have ran in the past that were done vertically. It took 30 minutes to let the gel bands separate before cracking off the plastic and letting it sit on the rocker over the weekend. The most important tip Chad gave us was to make sure the gel did not fold on itself.
Photo-bacterium Agar Broth; Making Media
3/10/18 For this week in lab, the initial process we learned was making a broth that would eventually have the bacterium added to it. We gathered all the materials and prepped 2 sizes of broth to create. The steps were simple as we weighed out each component carefully. The mixture was created then stirred on a hot plate with a stirring rod inside. This was then stored after proper labeling for later use. On the same day we performed a gram stain this process to me seemed to be one we will use often in lab. We followed the proper steps using heat fix then the 4 steps to staining. The results were the best part in which we saw results in the microscope. This was found to be gram positive.
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